Microbial Time-Kill Test

Test Purpose
The purpose of this microbiology lab test is to produce data that provides basic information on rate-of-kill of antimicrobial formulations tested against single selected microorganisms. This test is often used to characterize kill kinetics and substantiate basic efficacy of a wide range of antimicrobial products.

Test Summary
A suspension of the test organism is exposed to the test substance for specified contact times. After exposure, an aliquot of the suspension is transferred to a neutralizer and assayed for survivors. Appropriate culture purity, sterility, population and neutralization controls are performed.

Typical microbiology laboratory product development/screening tests include:

l  Microbial Time-Kill Tests

l  Minimum Inhibitory Concentration (MIC) Tests and

l  Minimum Bactericidal Concentration (MBC) Tests

l  Virucidal Suspension Tests

l  Virucidal Efficacy Tests

l  AOAC Use Dilution Test

l  AOAC Germicidal Spray Products Test

l  ASTM Pre-Saturated Towelettes Test

l  ASTM Non-Food Contact Sanitizer Test

l  AOAC Germicidal and Detergent Sanitizer Test

l  AOAC Tuberculocidal Activity Test

l  AOAC Sporicidal Activity Test