Virucidal Efficacy Tests

Test Purpose
The purpose of this test is to evaluate the virucidal efficacy of a client's liquid or spray product against a specific virus according to test criteria and methods approved by the U.S. Environmental Protection Agency for registration of a product as a virucide for hard, non-porous surfaces. The AOAC Use Dilution for Liquids microbiology lab test or the AOAC Germicidal Spray microbiology lab test must be conducted prior to running the virucidal efficacy test.

Test Summary
A film of virus, dried on a glass surface, is exposed to the test substance for a specified exposure time. Following exposure, the virucidal and cytotoxic activities are removed from the virus-test substance mixture, and the mixture is assayed for viral infectivity by an accepted assay method. Appropriate virus, test substance cytotoxicity, and neutralization controls are run concurrently.

Typical microbiology laboratory product development/screening tests include:

l  Microbial Time-Kill Tests

l  Minimum Inhibitory Concentration (MIC) Tests and

l  Minimum Bactericidal Concentration (MBC) Tests

l  Virucidal Suspension Tests

l  Virucidal Efficacy Tests

l  AOAC Use Dilution Test

l  AOAC Germicidal Spray Products Test

l  ASTM Pre-Saturated Towelettes Test

l  ASTM Non-Food Contact Sanitizer Test

l  AOAC Germicidal and Detergent Sanitizer Test

l  AOAC Tuberculocidal Activity Test

l  AOAC Sporicidal Activity Test